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prime script reverse transcriptase reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa prime script reverse transcriptase reagent kit
    Prime Script Reverse Transcriptase Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prime+script+reverse+transcriptase+reagent+kit/PrimeScript+Reverse+Transcriptase/10__3390_slash_ijms27104354-179-30-36
    Average 97 stars, based on 4907 article reviews
    prime script reverse transcriptase reagent kit - by Bioz Stars, 2026-09
    97/100 stars

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    Reverse Transcription:

    Article Title: Effects of Acrylamide on Mouse Implantation and Decidualization.
    Article Snippet: .. The total RNAs were extracted using the Trizol Reagent Kit (9109, Takara, Kusatsu, Japan), digested with RQ1 deoxyribonuclease I (Promega, Fitchburg, WI, USA), and reverse-transcribed into cDNA with the Prime Script Reverse Transcriptase Reagent Kit (Takara, Japan). .. For real-time PCR, the cDNA was amplified using a SYBR Premix Ex Taq Kit (Q311-02-AA, Vazyme, Nanjing, China) on the CFX96 Touch Realtime System (Bio-Rad, Irvine, CA, USA).

    Article Title: The activation of cGAS‐STING pathway causes abnormal uterine receptivity in aged mice
    Article Snippet: .. The total RNA was extracted using the Trizol Reagent Kit (9109, Takara, Japan), digested with RQ1 deoxyribonuclease I (Promega, Fitchburg, WI), and reverse‐transcribed into cDNA with the Prime Script Reverse Transcriptase Reagent Kit (Takara, Japan). .. For realtime PCR, the cDNA was amplified using a SYBR Premix Ex Taq Kit (TaKaRa) on the CFX96 Touch Realtime System (Bio‐Rad).

    Article Title: The activation of cGAS-STING pathway causes abnormal uterine receptivity in aged mice.
    Article Snippet: .. The total RNA was extracted using the Trizol Reagent Kit (9109, Takara, Japan), digested with RQ1 deoxyribonuclease I (Promega, Fitchburg, WI), and reverse- transcribed into cDNA with the Prime Script Reverse Transcriptase Reagent Kit (Takara, Japan). .. For realtime PCR, the cDNA was amplified using a SYBR Premix Ex Taq Kit (TaKaRa) on the CFX96 Touch Realtime System (Bio- Rad).

    Article Title: Vitamin D protects spermatogonia and Sertoli cells from heat stress damage by inhibiting NLRP3
    Article Snippet: .. The Trizol reagent and Prime Script reverse transcriptase reagent kit are from TaKaRa Biotechnology. .. GC-1 and TM4 cells were regularly cultured in DMEM/F12 (Gibco, USA) with 10% (v/v) FBS, 100 u/ml penicillin, and 100 mg/ml streptomycin in a humidified 37°C incubator with 5% CO2% and 95% air.

    Article Title: FOSL1 Mediates Collagen Synthesis and Myofibroblast Transformation in Aortic Fibroblasts Under Ang II-Induced Aortic Dissection Conditions.
    Article Snippet: .. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted from AFs using Trizol reagent (Ambion, 15596026) and reverse transcribed into Complementary DNA (cDNA) using a Prime Script reverse transcriptase reagent kit (Takara, RR037A). .. RT-qPCR analysis was performed using TB Green Premix Ex Taq (Takara, RR820A). β-actin served as an internal control for normalization.

    Article Title: Differential Expression and Function of Arginase in Mouse Uterus During Early Pregnancy
    Article Snippet: .. Total RNAs were extracted from mouse stromal cells using TRIzol (AG21101, Accurate Biology, Changsha, China), digested with RQ1 deoxyribonuclease I (Promega, Fitchburg, WI, USA), and reverse-transcribed into cDNA with the Prime Script Reverse Transcriptase Reagent Kit (Takara, Japan). .. RT-qPCR was performed using the SYBR Premix (Q311-02-AA, Vazyme, Nanjing, China).

    Article Title: Effects of Acrylamide on Mouse Implantation and Decidualization
    Article Snippet: .. The total RNAs were extracted using the Trizol Reagent Kit (9109, Takara, Kusatsu, Japan), digested with RQ1 deoxyribonuclease I (Promega, Fitchburg, WI, USA), and reverse-transcribed into cDNA with the Prime Script Reverse Transcriptase Reagent Kit (Takara, Japan). .. For real-time PCR, the cDNA was amplified using a SYBR Premix Ex Taq Kit (Q311-02-AA, Vazyme, Nanjing, China) on the CFX96 Touch Realtime System (Bio-Rad, Irvine, CA, USA).

    Article Title: Tryptophan in the mouse diet is essential for embryo implantation and decidualization
    Article Snippet: .. Total RNAs were digested with RQ1 deoxyribonuclease I (Promega, Fitchburg, WI) to remove DNA, and reverse-transcribed into cDNA with Prime Script Reverse Transcriptase Reagent Kit (Takara, Japan). .. Real-time PCR was performed using a SYBR Premix Ex Taq Kit (TaKaRa) on the CFX96 TouchTM Real-Time System (Bio-Rad) The 2-△△Ct method was used to calculate the data, which were standardized to the RPL7 (mouse) or RPL7 (human) level.

    Real-time Polymerase Chain Reaction:

    Article Title: FOSL1 Mediates Collagen Synthesis and Myofibroblast Transformation in Aortic Fibroblasts Under Ang II-Induced Aortic Dissection Conditions.
    Article Snippet: .. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted from AFs using Trizol reagent (Ambion, 15596026) and reverse transcribed into Complementary DNA (cDNA) using a Prime Script reverse transcriptase reagent kit (Takara, RR037A). .. RT-qPCR analysis was performed using TB Green Premix Ex Taq (Takara, RR820A). β-actin served as an internal control for normalization.



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